基于超高效液相色谱质谱联用仪的西红花有效成分分析研究Analysis and research of the effective components of saffron based on UPLC-MS
牛妍;张婷婷;崔丽阳;刘金彪;
摘要(Abstract):
以西红花粉末为研究对象,用超高效液相色谱质谱联用仪(ultra performance liquid chromatography-mass spectrometry,UPLC-MS)作为主要手段,确定了西红花有效成分的UPLC-MS条件。选用流动相为洗脱剂A (0.1%的甲酸水溶液,v/v),有机相为B (0.1%甲酸乙腈溶液,v/v),HSS T3柱(1.8μm×2.6 mm×100 mm)色谱柱,柱温为35℃,流速0.3 mL/min,进样量1μL, ESI离子源的正、负离子模式,正离子毛细管压为3.0 kV,负离子毛细管电压为2.0 kV。在此固定条件下,西红花溶液有效成分可以被准确地分离分析。经分析西红花中主要成分为山柰酚、藏红花酸、苦番红花素、西红花苷I和Ⅱ等10种化合物。为西红花质量控制研究奠定了基础。
关键词(KeyWords): 西红花;超高效液相色谱质谱联用仪;有效成分
基金项目(Foundation): 天津理工大学大学生创新训练计划项目(201810060129);; 天津市应用基础及前沿技术研究计划项目(15JCQNJC000)
作者(Authors): 牛妍;张婷婷;崔丽阳;刘金彪;
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