牛妍;张婷婷;崔丽阳;刘金彪;

• 1:天津理工大学化学化工学院
• 2:天津理工大学天津市药物靶向与生物成像重点实验室

摘要(Abstract)：

以西红花粉末为研究对象，用超高效液相色谱质谱联用仪（ultra performance liquid chromatography-mass spectrometry,UPLC-MS）作为主要手段，确定了西红花有效成分的UPLC-MS条件。选用流动相为洗脱剂A (0.1%的甲酸水溶液，v/v)，有机相为B (0.1%甲酸乙腈溶液，v/v),HSS T3柱（1.8μm×2.6 mm×100 mm）色谱柱，柱温为35℃，流速0.3 mL/min，进样量1μL, ESI离子源的正、负离子模式，正离子毛细管压为3.0 kV,负离子毛细管电压为2.0 kV。在此固定条件下，西红花溶液有效成分可以被准确地分离分析。经分析西红花中主要成分为山柰酚、藏红花酸、苦番红花素、西红花苷I和Ⅱ等10种化合物。为西红花质量控制研究奠定了基础。

关键词(KeyWords)： 西红花;超高效液相色谱质谱联用仪;有效成分

Abstract:

Keywords:

基金项目(Foundation): 天津理工大学大学生创新训练计划项目（201810060129）;; 天津市应用基础及前沿技术研究计划项目（15JCQNJC000）

作者(Authors): 牛妍;张婷婷;崔丽阳;刘金彪;

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